Private Sub TextBox1_KeyPress(ByVal sender As Object,ByVal e As System.Windows.Forms.KeyPressEventArgs)
                     Handles TextBox1.KeyPress,TextBox2.KeyPress,TextBox3.KeyPress
 
 
    '判断输入的是否是数字、小数点或“BackSpace”符号
 
    If Char.IsDigit(e.KeyChar) Or e.KeyChar = "." Or e.KeyChar = Chr(8) Then
 
         '判断输入的小数点是否唯一
          If e.KeyChar = "." And InStr(CType(sender,TextBox).Text,".") > 0 Then
               e.Handled = True
          Else
               e.Handled = False
          End If
 
     '判断输入的负号是否是在第一位
     ElseIf e.KeyChar = "-" And CType(sender,TextBox).Text = "" Then
          e.Handled = False
     Else
          e.Handled = True
     End If
End Sub

http://sheng.iteye.com/blog/720795