base参数。

一天是24小时，因此以12为基数的分组将从中午开始-中午。重新采样为您提供了介于两天之间的所有时间，因此如果您不需要完整的基础，可以.dropna(how='all')。 （我假设您有一个DatetimeIndex，如果没有，则可以使用resample的on参数指定日期时间列。）

df.resample('24H',base=12).mean()
#df.groupby(pd.Grouper(level=0,base=12,freq='24H')).mean() # Equivalent 

                         1      2          3
0                                           
2014-03-31 12:00:00  54.20  41.30  52.233333
2014-04-01 12:00:00  50.75  39.35  34.950000
2014-04-02 12:00:00    NaN    NaN        NaN
2014-04-03 12:00:00    NaN    NaN        NaN
2014-04-04 12:00:00    NaN    NaN        NaN
...                    ...    ...        ...
2016-11-26 12:00:00    NaN    NaN        NaN
2016-11-27 12:00:00    NaN    NaN        NaN
2016-11-28 12:00:00    NaN    NaN        NaN
2016-11-29 12:00:00    NaN    NaN        NaN
2016-11-30 12:00:00  17.80  15.45  40.450000

您可以减去时间和分组依据：

df.groupby((df.index - pd.to_timedelta('12:00:00')).normalize()).mean()

您可以将小时数更改12小时，然后按天重新采样。

from io import StringIO
import pandas as pd

data = """
2014-04-01 09:00:00,52.9,41.1,36.3
2014-04-01 10:00:00,56.4,41.6,70.8
2014-04-01 11:00:00,53.3,41.2,49.6
2014-04-01 12:00:00,50.4,39.5,36.6
2014-04-01 13:00:00,51.1,39.2,33.3
2016-11-30 16:00:00,16.0,13.5,36.6
2016-11-30 17:00:00,19.6,17.4,44.3
"""

df = pd.read_csv(StringIO(data),sep=',',header=None,index_col=0)

df.index = pd.to_datetime(df.index)
# shift by 12 hours
df.index = df.index - pd.Timedelta(hours=12)
# resample and drop na rows
df.resample('D').mean().dropna()